Batch Fractionation of Bovine Caseins with Diethylaminoethyl Cellulose
نویسندگان
چکیده
منابع مشابه
Thyroglobulin fractionation on diethylaminoethyl cellulose columns.
Thyroglobulin may be defined as the iodine-containing protein of the thyroid gland which has a sedimentation coefficient of 4o.w = 19 S, an electrophoretic mobility at pH 8.6 of approximately 5 x 10F5 cm2 volt-l set-1, and a steep salting out curve at about 38% ammonium sulfate saturation at neutral pH (1). Although other iodoproteins, both soluble in neutral salt and attached to cell particles...
متن کاملFractionation of Newcastle disease virus by chromatography on diethylaminoethyl cellulose.
Wilson, Dwight E. (Rensselaer Polytechnic Institute, Troy, N.Y.). Fractionation of Newcastle disease virus by chromatography on diethylaminoethyl cellulose. J. Bacteriol. 84:295-301. 1962.-The L. Kansas and NK strains of Newcastle disease virus were chromatographed on diethylaminoethyl (DEAE) cellulose ion-exchange columns. L. Kansas virus eluted from DEAE columns showed one peak of hemagglutin...
متن کاملChromatography of Myoglobin on Diethylaminoethyl Cellulose Columns*
Since the initial description (2, 3) of procedures for the use of cellulose ion exchange columns for the chromatography of proteins, a number of such systems have been developed for specific proteins, including the heme proteins. Thus, Gutter et al. (4) have described the use of carboxymethyl cellulose in a study of various hemoglobins, Rumen (5) has utilized carboxymethyl cellulose for the ide...
متن کاملElectrophoretic Analysis (Tricine-SDS-PAGE) of Bovine Caseins
An alternative electrophoretic method that improves the separation, identification and relative quantification of bovine milk casein fractions and casein hydrolysates was developed. The method is based in the Tricine-SDS-PAGE technique while the relative quantification of milk proteins has been achieved by digital densitometry. The advantages of this procedure for milk proteins and milk protein...
متن کاملFractionation of soluble ribonucleic acid on diethylaminoethyl Sephadex with a pH and salt gradient for elution.
A new method for the chromatographic fractionation of soluble ribonucleic acid (sRNA) is described. A gradient of increasing salt and pH is employed to elute sRNA from diethylaminoethyl Sephadex. The resolution of sRNA species for 10 different amino acids obtained with this method is presented. There is evidence that resolution is dependent upon a change in sRNA from a partially denatured state...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 1985
ISSN: 0022-0302
DOI: 10.3168/jds.s0022-0302(85)81006-7